cell culture supernatants Search Results


cell culture supernatants elisa max sandwich elisa kits  (Revvity)
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Revvity cell culture supernatants elisa max sandwich elisa kits
Cell Culture Supernatants Elisa Max Sandwich Elisa Kits, supplied by Revvity, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csb ei0102  (Cusabio)
90
Cusabio csb ei0102
Csb Ei0102, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mn12h2 hybridoma cell-culture supernatant  (Bilthoven Biologicals)
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Bilthoven Biologicals mn12h2 hybridoma cell-culture supernatant
Mn12h2 Hybridoma Cell Culture Supernatant, supplied by Bilthoven Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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single cell culture supernatant screening  (10X Genomics)
90
10X Genomics single cell culture supernatant screening
Single Cell Culture Supernatant Screening, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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single cell culture supernatant screening - by Bioz Stars, 2026-02
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mouse igm purified from the tissue culture supernatant of a mouse myeloma cell line  (Cappel Laboratories)
90
Cappel Laboratories mouse igm purified from the tissue culture supernatant of a mouse myeloma cell line
Mouse Igm Purified From The Tissue Culture Supernatant Of A Mouse Myeloma Cell Line, supplied by Cappel Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse igm purified from the tissue culture supernatant of a mouse myeloma cell line - by Bioz Stars, 2026-02
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native commercially available laminin 332 purified by affinity chromatography from cell culture supernatant of human foreskin keratinocytes  (Immundiagnostik AG)
90
Immundiagnostik AG native commercially available laminin 332 purified by affinity chromatography from cell culture supernatant of human foreskin keratinocytes
Detection of autoantibodies by ELISA with extracellular matrix of cultured <t>keratinocytes.</t> The extracellular matrix was obtained by treatment of cultured HaCaT keratinocytes with 20 mM NH4OH as detailed in Methods. a SDS-PAGE analysis of the extracellular matrix extract shows the migration of laminin 332 chains (arrows) at about 165 kDa (α3 chain), 140 kDa (β3 chain) as well as 155 kDa and 105 kDa (unprocessed and processed forms of the γ2 chain). b Immunoreactivity of mucous membrane pemphigoid autoantibodies with the laminin 332 from the extracellular matrix extract. Extracellular matrix extract was electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patient’s sera (lanes 2–4), normal human sera (lane 5), and normal rabbit sera (lane 6). c Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid ( n = 36) and controls ( n = 116). d ELISA reactivity of human sera with extracellular matrix. Scatter plots represent optical density measurements of serum reactivity from patients with mucous membrane pemphigoid (MMP; n = 36) with confirmed laminin 332-specific autoantibodies, MMP patients with possible laminin 332-specific autoantibodies ( n = 30), from healthy donors (NHS; n = 116), as well as from patients with bullous pemphigoid (BP; n = 89), pemphigus vulgaris (PV; n = 49), epidermolysis bullosa acquisita (EBA; n = 19), and dermatitis herpetiformis (DH; n = 41). The cut-off of the assay is represented by a dotted line (cut-off = 0.367, sensitivity = 83.33%, specificity = 84.48%)
Native Commercially Available Laminin 332 Purified By Affinity Chromatography From Cell Culture Supernatant Of Human Foreskin Keratinocytes, supplied by Immundiagnostik AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hev-sg produced from clarified cho cell culture supernatant  (Zoetis)
90
Zoetis hev-sg produced from clarified cho cell culture supernatant
Detection of autoantibodies by ELISA with extracellular matrix of cultured <t>keratinocytes.</t> The extracellular matrix was obtained by treatment of cultured HaCaT keratinocytes with 20 mM NH4OH as detailed in Methods. a SDS-PAGE analysis of the extracellular matrix extract shows the migration of laminin 332 chains (arrows) at about 165 kDa (α3 chain), 140 kDa (β3 chain) as well as 155 kDa and 105 kDa (unprocessed and processed forms of the γ2 chain). b Immunoreactivity of mucous membrane pemphigoid autoantibodies with the laminin 332 from the extracellular matrix extract. Extracellular matrix extract was electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patient’s sera (lanes 2–4), normal human sera (lane 5), and normal rabbit sera (lane 6). c Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid ( n = 36) and controls ( n = 116). d ELISA reactivity of human sera with extracellular matrix. Scatter plots represent optical density measurements of serum reactivity from patients with mucous membrane pemphigoid (MMP; n = 36) with confirmed laminin 332-specific autoantibodies, MMP patients with possible laminin 332-specific autoantibodies ( n = 30), from healthy donors (NHS; n = 116), as well as from patients with bullous pemphigoid (BP; n = 89), pemphigus vulgaris (PV; n = 49), epidermolysis bullosa acquisita (EBA; n = 19), and dermatitis herpetiformis (DH; n = 41). The cut-off of the assay is represented by a dotted line (cut-off = 0.367, sensitivity = 83.33%, specificity = 84.48%)
Hev Sg Produced From Clarified Cho Cell Culture Supernatant, supplied by Zoetis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fm cell culture supernatants  (Cellbox Solutions)
90
Cellbox Solutions fm cell culture supernatants
Detection of autoantibodies by ELISA with extracellular matrix of cultured <t>keratinocytes.</t> The extracellular matrix was obtained by treatment of cultured HaCaT keratinocytes with 20 mM NH4OH as detailed in Methods. a SDS-PAGE analysis of the extracellular matrix extract shows the migration of laminin 332 chains (arrows) at about 165 kDa (α3 chain), 140 kDa (β3 chain) as well as 155 kDa and 105 kDa (unprocessed and processed forms of the γ2 chain). b Immunoreactivity of mucous membrane pemphigoid autoantibodies with the laminin 332 from the extracellular matrix extract. Extracellular matrix extract was electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patient’s sera (lanes 2–4), normal human sera (lane 5), and normal rabbit sera (lane 6). c Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid ( n = 36) and controls ( n = 116). d ELISA reactivity of human sera with extracellular matrix. Scatter plots represent optical density measurements of serum reactivity from patients with mucous membrane pemphigoid (MMP; n = 36) with confirmed laminin 332-specific autoantibodies, MMP patients with possible laminin 332-specific autoantibodies ( n = 30), from healthy donors (NHS; n = 116), as well as from patients with bullous pemphigoid (BP; n = 89), pemphigus vulgaris (PV; n = 49), epidermolysis bullosa acquisita (EBA; n = 19), and dermatitis herpetiformis (DH; n = 41). The cut-off of the assay is represented by a dotted line (cut-off = 0.367, sensitivity = 83.33%, specificity = 84.48%)
Fm Cell Culture Supernatants, supplied by Cellbox Solutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fm cell culture supernatants - by Bioz Stars, 2026-02
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exosomes from cell culture supernatant  (HansaBioMed ltd)
90
HansaBioMed ltd exosomes from cell culture supernatant
Detection of autoantibodies by ELISA with extracellular matrix of cultured <t>keratinocytes.</t> The extracellular matrix was obtained by treatment of cultured HaCaT keratinocytes with 20 mM NH4OH as detailed in Methods. a SDS-PAGE analysis of the extracellular matrix extract shows the migration of laminin 332 chains (arrows) at about 165 kDa (α3 chain), 140 kDa (β3 chain) as well as 155 kDa and 105 kDa (unprocessed and processed forms of the γ2 chain). b Immunoreactivity of mucous membrane pemphigoid autoantibodies with the laminin 332 from the extracellular matrix extract. Extracellular matrix extract was electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patient’s sera (lanes 2–4), normal human sera (lane 5), and normal rabbit sera (lane 6). c Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid ( n = 36) and controls ( n = 116). d ELISA reactivity of human sera with extracellular matrix. Scatter plots represent optical density measurements of serum reactivity from patients with mucous membrane pemphigoid (MMP; n = 36) with confirmed laminin 332-specific autoantibodies, MMP patients with possible laminin 332-specific autoantibodies ( n = 30), from healthy donors (NHS; n = 116), as well as from patients with bullous pemphigoid (BP; n = 89), pemphigus vulgaris (PV; n = 49), epidermolysis bullosa acquisita (EBA; n = 19), and dermatitis herpetiformis (DH; n = 41). The cut-off of the assay is represented by a dotted line (cut-off = 0.367, sensitivity = 83.33%, specificity = 84.48%)
Exosomes From Cell Culture Supernatant, supplied by HansaBioMed ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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exosomes from cell culture supernatant - by Bioz Stars, 2026-02
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lyophilized exosome standard from colo-1 cell culture supernatant (2×30 μg vials)  (HansaBioMed ltd)
90
HansaBioMed ltd lyophilized exosome standard from colo-1 cell culture supernatant (2×30 μg vials)
Detection of autoantibodies by ELISA with extracellular matrix of cultured <t>keratinocytes.</t> The extracellular matrix was obtained by treatment of cultured HaCaT keratinocytes with 20 mM NH4OH as detailed in Methods. a SDS-PAGE analysis of the extracellular matrix extract shows the migration of laminin 332 chains (arrows) at about 165 kDa (α3 chain), 140 kDa (β3 chain) as well as 155 kDa and 105 kDa (unprocessed and processed forms of the γ2 chain). b Immunoreactivity of mucous membrane pemphigoid autoantibodies with the laminin 332 from the extracellular matrix extract. Extracellular matrix extract was electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patient’s sera (lanes 2–4), normal human sera (lane 5), and normal rabbit sera (lane 6). c Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid ( n = 36) and controls ( n = 116). d ELISA reactivity of human sera with extracellular matrix. Scatter plots represent optical density measurements of serum reactivity from patients with mucous membrane pemphigoid (MMP; n = 36) with confirmed laminin 332-specific autoantibodies, MMP patients with possible laminin 332-specific autoantibodies ( n = 30), from healthy donors (NHS; n = 116), as well as from patients with bullous pemphigoid (BP; n = 89), pemphigus vulgaris (PV; n = 49), epidermolysis bullosa acquisita (EBA; n = 19), and dermatitis herpetiformis (DH; n = 41). The cut-off of the assay is represented by a dotted line (cut-off = 0.367, sensitivity = 83.33%, specificity = 84.48%)
Lyophilized Exosome Standard From Colo 1 Cell Culture Supernatant (2×30 μg Vials), supplied by HansaBioMed ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lyophilized exosome standard from colo-1 cell culture supernatant (2×30 μg vials) - by Bioz Stars, 2026-02
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293 cell culture supernatant  (Trommsdorff)
90
Trommsdorff 293 cell culture supernatant
Detection of autoantibodies by ELISA with extracellular matrix of cultured <t>keratinocytes.</t> The extracellular matrix was obtained by treatment of cultured HaCaT keratinocytes with 20 mM NH4OH as detailed in Methods. a SDS-PAGE analysis of the extracellular matrix extract shows the migration of laminin 332 chains (arrows) at about 165 kDa (α3 chain), 140 kDa (β3 chain) as well as 155 kDa and 105 kDa (unprocessed and processed forms of the γ2 chain). b Immunoreactivity of mucous membrane pemphigoid autoantibodies with the laminin 332 from the extracellular matrix extract. Extracellular matrix extract was electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patient’s sera (lanes 2–4), normal human sera (lane 5), and normal rabbit sera (lane 6). c Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid ( n = 36) and controls ( n = 116). d ELISA reactivity of human sera with extracellular matrix. Scatter plots represent optical density measurements of serum reactivity from patients with mucous membrane pemphigoid (MMP; n = 36) with confirmed laminin 332-specific autoantibodies, MMP patients with possible laminin 332-specific autoantibodies ( n = 30), from healthy donors (NHS; n = 116), as well as from patients with bullous pemphigoid (BP; n = 89), pemphigus vulgaris (PV; n = 49), epidermolysis bullosa acquisita (EBA; n = 19), and dermatitis herpetiformis (DH; n = 41). The cut-off of the assay is represented by a dotted line (cut-off = 0.367, sensitivity = 83.33%, specificity = 84.48%)
293 Cell Culture Supernatant, supplied by Trommsdorff, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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293 cell culture supernatant - by Bioz Stars, 2026-02
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strem2 elisa for quantitation of levels of strem2 in cell culture supernatants, an elisa for human strem2  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC strem2 elisa for quantitation of levels of strem2 in cell culture supernatants, an elisa for human strem2
Detection of autoantibodies by ELISA with extracellular matrix of cultured <t>keratinocytes.</t> The extracellular matrix was obtained by treatment of cultured HaCaT keratinocytes with 20 mM NH4OH as detailed in Methods. a SDS-PAGE analysis of the extracellular matrix extract shows the migration of laminin 332 chains (arrows) at about 165 kDa (α3 chain), 140 kDa (β3 chain) as well as 155 kDa and 105 kDa (unprocessed and processed forms of the γ2 chain). b Immunoreactivity of mucous membrane pemphigoid autoantibodies with the laminin 332 from the extracellular matrix extract. Extracellular matrix extract was electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patient’s sera (lanes 2–4), normal human sera (lane 5), and normal rabbit sera (lane 6). c Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid ( n = 36) and controls ( n = 116). d ELISA reactivity of human sera with extracellular matrix. Scatter plots represent optical density measurements of serum reactivity from patients with mucous membrane pemphigoid (MMP; n = 36) with confirmed laminin 332-specific autoantibodies, MMP patients with possible laminin 332-specific autoantibodies ( n = 30), from healthy donors (NHS; n = 116), as well as from patients with bullous pemphigoid (BP; n = 89), pemphigus vulgaris (PV; n = 49), epidermolysis bullosa acquisita (EBA; n = 19), and dermatitis herpetiformis (DH; n = 41). The cut-off of the assay is represented by a dotted line (cut-off = 0.367, sensitivity = 83.33%, specificity = 84.48%)
Strem2 Elisa For Quantitation Of Levels Of Strem2 In Cell Culture Supernatants, An Elisa For Human Strem2, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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strem2 elisa for quantitation of levels of strem2 in cell culture supernatants, an elisa for human strem2 - by Bioz Stars, 2026-02
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Detection of autoantibodies by ELISA with extracellular matrix of cultured keratinocytes. The extracellular matrix was obtained by treatment of cultured HaCaT keratinocytes with 20 mM NH4OH as detailed in Methods. a SDS-PAGE analysis of the extracellular matrix extract shows the migration of laminin 332 chains (arrows) at about 165 kDa (α3 chain), 140 kDa (β3 chain) as well as 155 kDa and 105 kDa (unprocessed and processed forms of the γ2 chain). b Immunoreactivity of mucous membrane pemphigoid autoantibodies with the laminin 332 from the extracellular matrix extract. Extracellular matrix extract was electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patient’s sera (lanes 2–4), normal human sera (lane 5), and normal rabbit sera (lane 6). c Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid ( n = 36) and controls ( n = 116). d ELISA reactivity of human sera with extracellular matrix. Scatter plots represent optical density measurements of serum reactivity from patients with mucous membrane pemphigoid (MMP; n = 36) with confirmed laminin 332-specific autoantibodies, MMP patients with possible laminin 332-specific autoantibodies ( n = 30), from healthy donors (NHS; n = 116), as well as from patients with bullous pemphigoid (BP; n = 89), pemphigus vulgaris (PV; n = 49), epidermolysis bullosa acquisita (EBA; n = 19), and dermatitis herpetiformis (DH; n = 41). The cut-off of the assay is represented by a dotted line (cut-off = 0.367, sensitivity = 83.33%, specificity = 84.48%)

Journal: Orphanet Journal of Rare Diseases

Article Title: Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid

doi: 10.1186/s13023-018-0855-x

Figure Lengend Snippet: Detection of autoantibodies by ELISA with extracellular matrix of cultured keratinocytes. The extracellular matrix was obtained by treatment of cultured HaCaT keratinocytes with 20 mM NH4OH as detailed in Methods. a SDS-PAGE analysis of the extracellular matrix extract shows the migration of laminin 332 chains (arrows) at about 165 kDa (α3 chain), 140 kDa (β3 chain) as well as 155 kDa and 105 kDa (unprocessed and processed forms of the γ2 chain). b Immunoreactivity of mucous membrane pemphigoid autoantibodies with the laminin 332 from the extracellular matrix extract. Extracellular matrix extract was electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patient’s sera (lanes 2–4), normal human sera (lane 5), and normal rabbit sera (lane 6). c Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid ( n = 36) and controls ( n = 116). d ELISA reactivity of human sera with extracellular matrix. Scatter plots represent optical density measurements of serum reactivity from patients with mucous membrane pemphigoid (MMP; n = 36) with confirmed laminin 332-specific autoantibodies, MMP patients with possible laminin 332-specific autoantibodies ( n = 30), from healthy donors (NHS; n = 116), as well as from patients with bullous pemphigoid (BP; n = 89), pemphigus vulgaris (PV; n = 49), epidermolysis bullosa acquisita (EBA; n = 19), and dermatitis herpetiformis (DH; n = 41). The cut-off of the assay is represented by a dotted line (cut-off = 0.367, sensitivity = 83.33%, specificity = 84.48%)

Article Snippet: Native commercially available laminin 332 purified by affinity chromatography from cell culture supernatant of human foreskin keratinocytes has been purchased from Immundiagnostik.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, SDS Page, Migration

Use of laminin 332-specific immunoassays in the diagnostic algorithm in mucous membrane pemphigoid. The ELISA with extracellular matrix could represent an addition in patients with a high index of clinical suspicion of mucous membrane pemphigoid showing compatible results by direct immunofluorescence microscopy and either negative or positive findings with dermal binding by indirect immunofluorescence microscopy. A positive result by ELISA with keratinocyte extracellular matrix should be subsequently confirmed by immunoblotting

Journal: Orphanet Journal of Rare Diseases

Article Title: Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid

doi: 10.1186/s13023-018-0855-x

Figure Lengend Snippet: Use of laminin 332-specific immunoassays in the diagnostic algorithm in mucous membrane pemphigoid. The ELISA with extracellular matrix could represent an addition in patients with a high index of clinical suspicion of mucous membrane pemphigoid showing compatible results by direct immunofluorescence microscopy and either negative or positive findings with dermal binding by indirect immunofluorescence microscopy. A positive result by ELISA with keratinocyte extracellular matrix should be subsequently confirmed by immunoblotting

Article Snippet: Native commercially available laminin 332 purified by affinity chromatography from cell culture supernatant of human foreskin keratinocytes has been purchased from Immundiagnostik.

Techniques: Diagnostic Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Microscopy, Binding Assay, Western Blot